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Stabilising G-quadruplex
Project Details
A. Brief Description of Project
Using various spectroscopy techniques such as Nuclear Magnetic Resonance (NMR), we tried to find out the structures of a certain section of a DNA sequence, specifically the telemere region of the sequence. The sequences we examined were vital because they were found to be the major cause of cancer production in humans. Finding a way to stabilize them would be essential in creating a cure for cancer.
B. Results
We observed that when we change the conditions of the G-quadruplex structure formed, the electrons will be found in different parts of the structures. We are able to tell this by observing the peaks on the graph we obtained from NMR. We can change the conditions of the G-quadruplex structure by putting the substance in potassium buffer or lithium buffer to alter the salt content of the G-quadruplex. We can also change the forming of the G-quadruplex structure through quenching and annealing (fast heating or slow heating).
C. Reflection
Three content knowledge/skills that we have learnt
1. We learnt how to use the NMR machine.
2. We learnt that the DNA structure can be altered by varying the conditions they are in.
3. We learnt how to make a DNA sequence.
Two interesting aspects about our learning
1. We get to use a variety of expensive and world-class machines
2.
One take-away for life
1. We must have passion in our work to go far.
Gallery
Insert photos (in jpg, gif format), videos (using Youtube) or animation here.For files such as animations or online games that are larger than 2 MB, please pass them to Mr Tan Seng Kwang directly in the form of a CD and he will put up the link for you.
e.g. to insert photo please save them in jpg or gif formats that are at most 400 pixels in width and height, go to "Image" in the EasyEdit Toolbar.
e.g. to insert video, go to "Widget (video, rss)" in the EasyEdit Toolbar. Make sure you have uploaded the video in either Youtube or GoogleVideo.
e.g. to insert links to existing websites/blogs, click on "Link" in the EasyEdit Toolbar.
| Organisation: | NTU School of Physical and Mathematical Sciences |
| Mentor: | Prof Phan Ahn Tuan, Dr Samir |
| Members: | Mahendran Balaji, Aw Zhan Quan |
| Project Title: | Stabilising G-quadruplex |
A. Brief Description of Project
Using various spectroscopy techniques such as Nuclear Magnetic Resonance (NMR), we tried to find out the structures of a certain section of a DNA sequence, specifically the telemere region of the sequence. The sequences we examined were vital because they were found to be the major cause of cancer production in humans. Finding a way to stabilize them would be essential in creating a cure for cancer.
B. Results
We observed that when we change the conditions of the G-quadruplex structure formed, the electrons will be found in different parts of the structures. We are able to tell this by observing the peaks on the graph we obtained from NMR. We can change the conditions of the G-quadruplex structure by putting the substance in potassium buffer or lithium buffer to alter the salt content of the G-quadruplex. We can also change the forming of the G-quadruplex structure through quenching and annealing (fast heating or slow heating).
C. Reflection
Three content knowledge/skills that we have learnt
1. We learnt how to use the NMR machine.
2. We learnt that the DNA structure can be altered by varying the conditions they are in.
3. We learnt how to make a DNA sequence.
Two interesting aspects about our learning
1. We get to use a variety of expensive and world-class machines
2.
One take-away for life
1. We must have passion in our work to go far.
Gallery
Insert photos (in jpg, gif format), videos (using Youtube) or animation here.For files such as animations or online games that are larger than 2 MB, please pass them to Mr Tan Seng Kwang directly in the form of a CD and he will put up the link for you.
e.g. to insert photo please save them in jpg or gif formats that are at most 400 pixels in width and height, go to "Image" in the EasyEdit Toolbar.
e.g. to insert video, go to "Widget (video, rss)" in the EasyEdit Toolbar. Make sure you have uploaded the video in either Youtube or GoogleVideo.
e.g. to insert links to existing websites/blogs, click on "Link" in the EasyEdit Toolbar.
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Latest page update: made by tanchorkmeng
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